Mouse SPH ELISA Kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Sphingomyelin (SPH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Sphingomyelin (SPH) and unlabeled Sphingomyelin (SPH) (Standards or samples) with the pre-coated antibody specific to Sphingomyelin (SPH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Sphingomyelin (SPH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Sphingomyelin (SPH) in the sample. Species : Mouse Storage: For 5-7days:Store the whole kit at 4℃For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃. Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids. Gene: SPH Uniprot AC: Intended Use: Mouse SPH ELISA Kitallows for the in vitro quantitative determination of SPH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.