Mouse Wnt8b ELISA Kit

Principle of the Assay:This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT8B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT8B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT8B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT8B bound in the initial step. The color development is stopped and the intensity of the color is measured. Species : Mouse Storage: Store the whole ELISA kit at 4℃ Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids. Gene: Wnt8b Uniprot AC: Q9WUD6; Intended Use: Mouse Wnt8b ELISA Kitallows for the in vitro quantitative determination of Wnt8b , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.