Mouse Zg16 ELISA Kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zymogen granule protein 16. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Zymogen granule protein 16 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Zymogen granule protein 16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Zymogen granule protein 16 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Species : Mouse Storage: Store the whole ELISA kit at 4℃ Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids. Gene: Zg16 Uniprot AC: Q8K0C5; Intended Use: Mouse Zg16 ELISA Kitallows for the in vitro quantitative determination of Zg16 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.