Mouse Caspase-12 ELISA Kit

Principle of the assay: Caspase 12 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Caspase 12 antibodies and an Caspase 12-HRP conjugate. The assay sample and buffer are incubated together with Caspase 12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Caspase 12 concentration since Caspase 12 from samples and Caspase 12-HRP conjugate compete for the anti-Caspase 12 antibody binding site. Since the number of sites is limited, as more sites are occupied by Caspase 12 from the sample, fewer sites are left to bind Caspase 12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Caspase 12 concentration in each sample is interpolated from this standard curve. Species : Mouse Storage: For 5-7days:Store the whole kit at 4℃For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃. Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids. Gene: Caspase-12 Uniprot AC: Intended Use: Mouse Caspase-12 ELISA Kitallows for the in vitro quantitative determination of Caspase-12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.